bio rad unoq Search Results


uno q column  (Bio-Rad)
90
Bio-Rad uno q column
Uno Q Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
uno q column - by Bioz Stars, 2026-02
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unoq column  (Bio-Rad)
90
Bio-Rad unoq column
Unoq Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/unoq column/product/Bio-Rad
Average 90 stars, based on 1 article reviews
unoq column - by Bioz Stars, 2026-02
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uno q6 anion exchange column  (Bio-Rad)
93
Bio-Rad uno q6 anion exchange column
Uno Q6 Anion Exchange Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
uno q6 anion exchange column - by Bioz Stars, 2026-02
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bio rad uno s6 cation exchange column  (Bio-Rad)
93
Bio-Rad bio rad uno s6 cation exchange column
Bio Rad Uno S6 Cation Exchange Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
bio rad uno s6 cation exchange column - by Bioz Stars, 2026-02
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bu¡er uno a  (Bio-Rad)
96
Bio-Rad bu¡er uno a
Fig. 1. Overlay plots showing the binding of core RNA polymerase to His-tagged c70 immobilised on the Ni2^NTA surface. The £ow rate was 20 Wl/min. The signal for binding of the ligand (c70) was 500 RU. Core polymerase was passed over the chip for about 100 s, before initiating the dissociation phase by washing with <t>bu¡er.</t> The polymerase concentrations were 1.05 WM (a and b), 0.525 WM (c), 0.21 WM (d) and 0.105 WM (e). Kinetic analysis was carried out on the dissociation phases of the traces, yielding dissociation rate constants of: (a) 4.6( þ 0.5)U1033 s31, (b) 4.2( þ 0.5)U1033 s31, (c) 4.2( þ 0.7)U1033 s31, (d) 3.9( þ 1.0)U1033 s31, (e) 3.6( þ 1.5)U 1033 s31.
Bu¡Er Uno A, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bu¡er uno a/product/Bio-Rad
Average 96 stars, based on 1 article reviews
bu¡er uno a - by Bioz Stars, 2026-02
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unoq12 column  (Bio-Rad)
94
Bio-Rad unoq12 column
Fig. 1. Overlay plots showing the binding of core RNA polymerase to His-tagged c70 immobilised on the Ni2^NTA surface. The £ow rate was 20 Wl/min. The signal for binding of the ligand (c70) was 500 RU. Core polymerase was passed over the chip for about 100 s, before initiating the dissociation phase by washing with <t>bu¡er.</t> The polymerase concentrations were 1.05 WM (a and b), 0.525 WM (c), 0.21 WM (d) and 0.105 WM (e). Kinetic analysis was carried out on the dissociation phases of the traces, yielding dissociation rate constants of: (a) 4.6( þ 0.5)U1033 s31, (b) 4.2( þ 0.5)U1033 s31, (c) 4.2( þ 0.7)U1033 s31, (d) 3.9( þ 1.0)U1033 s31, (e) 3.6( þ 1.5)U 1033 s31.
Unoq12 Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
unoq12 column - by Bioz Stars, 2026-02
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1.3-ml uno™ q ion exchange column  (Bio-Rad)
90
Bio-Rad 1.3-ml uno™ q ion exchange column
Fig. 1. Overlay plots showing the binding of core RNA polymerase to His-tagged c70 immobilised on the Ni2^NTA surface. The £ow rate was 20 Wl/min. The signal for binding of the ligand (c70) was 500 RU. Core polymerase was passed over the chip for about 100 s, before initiating the dissociation phase by washing with <t>bu¡er.</t> The polymerase concentrations were 1.05 WM (a and b), 0.525 WM (c), 0.21 WM (d) and 0.105 WM (e). Kinetic analysis was carried out on the dissociation phases of the traces, yielding dissociation rate constants of: (a) 4.6( þ 0.5)U1033 s31, (b) 4.2( þ 0.5)U1033 s31, (c) 4.2( þ 0.7)U1033 s31, (d) 3.9( þ 1.0)U1033 s31, (e) 3.6( þ 1.5)U 1033 s31.
1.3 Ml Uno™ Q Ion Exchange Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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uno s1 fplc column  (Bio-Rad)
93
Bio-Rad uno s1 fplc column
Fig. 1. Overlay plots showing the binding of core RNA polymerase to His-tagged c70 immobilised on the Ni2^NTA surface. The £ow rate was 20 Wl/min. The signal for binding of the ligand (c70) was 500 RU. Core polymerase was passed over the chip for about 100 s, before initiating the dissociation phase by washing with <t>bu¡er.</t> The polymerase concentrations were 1.05 WM (a and b), 0.525 WM (c), 0.21 WM (d) and 0.105 WM (e). Kinetic analysis was carried out on the dissociation phases of the traces, yielding dissociation rate constants of: (a) 4.6( þ 0.5)U1033 s31, (b) 4.2( þ 0.5)U1033 s31, (c) 4.2( þ 0.7)U1033 s31, (d) 3.9( þ 1.0)U1033 s31, (e) 3.6( þ 1.5)U 1033 s31.
Uno S1 Fplc Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
uno s1 fplc column - by Bioz Stars, 2026-02
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cation exchange column  (Bio-Rad)
88
Bio-Rad cation exchange column
Fig. 1. Overlay plots showing the binding of core RNA polymerase to His-tagged c70 immobilised on the Ni2^NTA surface. The £ow rate was 20 Wl/min. The signal for binding of the ligand (c70) was 500 RU. Core polymerase was passed over the chip for about 100 s, before initiating the dissociation phase by washing with <t>bu¡er.</t> The polymerase concentrations were 1.05 WM (a and b), 0.525 WM (c), 0.21 WM (d) and 0.105 WM (e). Kinetic analysis was carried out on the dissociation phases of the traces, yielding dissociation rate constants of: (a) 4.6( þ 0.5)U1033 s31, (b) 4.2( þ 0.5)U1033 s31, (c) 4.2( þ 0.7)U1033 s31, (d) 3.9( þ 1.0)U1033 s31, (e) 3.6( þ 1.5)U 1033 s31.
Cation Exchange Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 88 stars, based on 1 article reviews
cation exchange column - by Bioz Stars, 2026-02
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uno s column  (Bio-Rad)
93
Bio-Rad uno s column
Fig. 1. Overlay plots showing the binding of core RNA polymerase to His-tagged c70 immobilised on the Ni2^NTA surface. The £ow rate was 20 Wl/min. The signal for binding of the ligand (c70) was 500 RU. Core polymerase was passed over the chip for about 100 s, before initiating the dissociation phase by washing with <t>bu¡er.</t> The polymerase concentrations were 1.05 WM (a and b), 0.525 WM (c), 0.21 WM (d) and 0.105 WM (e). Kinetic analysis was carried out on the dissociation phases of the traces, yielding dissociation rate constants of: (a) 4.6( þ 0.5)U1033 s31, (b) 4.2( þ 0.5)U1033 s31, (c) 4.2( þ 0.7)U1033 s31, (d) 3.9( þ 1.0)U1033 s31, (e) 3.6( þ 1.5)U 1033 s31.
Uno S Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
uno s column - by Bioz Stars, 2026-02
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uno-q  (Bio-Rad)
90
Bio-Rad uno-q
Fig. 1. Overlay plots showing the binding of core RNA polymerase to His-tagged c70 immobilised on the Ni2^NTA surface. The £ow rate was 20 Wl/min. The signal for binding of the ligand (c70) was 500 RU. Core polymerase was passed over the chip for about 100 s, before initiating the dissociation phase by washing with <t>bu¡er.</t> The polymerase concentrations were 1.05 WM (a and b), 0.525 WM (c), 0.21 WM (d) and 0.105 WM (e). Kinetic analysis was carried out on the dissociation phases of the traces, yielding dissociation rate constants of: (a) 4.6( þ 0.5)U1033 s31, (b) 4.2( þ 0.5)U1033 s31, (c) 4.2( þ 0.7)U1033 s31, (d) 3.9( þ 1.0)U1033 s31, (e) 3.6( þ 1.5)U 1033 s31.
Uno Q, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/uno-q/product/Bio-Rad
Average 90 stars, based on 1 article reviews
uno-q - by Bioz Stars, 2026-02
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Image Search Results


Fig. 1. Overlay plots showing the binding of core RNA polymerase to His-tagged c70 immobilised on the Ni2^NTA surface. The £ow rate was 20 Wl/min. The signal for binding of the ligand (c70) was 500 RU. Core polymerase was passed over the chip for about 100 s, before initiating the dissociation phase by washing with bu¡er. The polymerase concentrations were 1.05 WM (a and b), 0.525 WM (c), 0.21 WM (d) and 0.105 WM (e). Kinetic analysis was carried out on the dissociation phases of the traces, yielding dissociation rate constants of: (a) 4.6( þ 0.5)U1033 s31, (b) 4.2( þ 0.5)U1033 s31, (c) 4.2( þ 0.7)U1033 s31, (d) 3.9( þ 1.0)U1033 s31, (e) 3.6( þ 1.5)U 1033 s31.

Journal: FEBS letters

Article Title: Interaction of sigma 70 with Escherichia coli RNA polymerase core enzyme studied by surface plasmon resonance.

doi: 10.1016/s0014-5793(00)02028-7

Figure Lengend Snippet: Fig. 1. Overlay plots showing the binding of core RNA polymerase to His-tagged c70 immobilised on the Ni2^NTA surface. The £ow rate was 20 Wl/min. The signal for binding of the ligand (c70) was 500 RU. Core polymerase was passed over the chip for about 100 s, before initiating the dissociation phase by washing with bu¡er. The polymerase concentrations were 1.05 WM (a and b), 0.525 WM (c), 0.21 WM (d) and 0.105 WM (e). Kinetic analysis was carried out on the dissociation phases of the traces, yielding dissociation rate constants of: (a) 4.6( þ 0.5)U1033 s31, (b) 4.2( þ 0.5)U1033 s31, (c) 4.2( þ 0.7)U1033 s31, (d) 3.9( þ 1.0)U1033 s31, (e) 3.6( þ 1.5)U 1033 s31.

Article Snippet: Fractions containing c70 were pooled, dialysed into bu¡er Uno-A (10 mM Tris^HCl, pH 8.0, 5% glycerol, 1 mM EDTA, 0.1 mM DTT, 50 mM NaCl), and applied to an UnoQ-6R column (Bio-Rad) and eluted with a linear gradient of bu¡er Uno-A and bu¡er Uno-B (bu¡er Uno-A with 1 M NaCl).

Techniques: Binding Assay